Effects of 7-minute practices of breathing and meditation on stress reduction.

We compared the effects of 7-min practices of breathing and meditation on perceived stress reduction and related affective outcomes (active emotion, serenity, anxiety, and fatigue) during micro-breaks. Undergraduates from two classes (N = 59) completed the 7-point online surveys. Results supported the effects of both practices.

CircularRNA Hsa_circ_0093335 promotes hepatocellular carcinoma progression via sponging miR-338-5p.

Circular RNAs play an important role in the development of various malignancies, including hepatocellular carcinoma (HCC). Nevertheless, the role of Hsa_circ_0093335 (circ0093335) in HCC has not yet been explored. To investigate the biological effects and molecular mechanisms of circ0093335 on HCC. Circ0093335 expression was detected in HCC cells and clinical specimens using qRT-PCR. The association between circ0093335 expression and HCC patients' clinical characteristics was determined using SPSS. The role of circ0093335 in HCC was estimated by overexpression and knockdown experiments in vitro and in vivo. qRT-PCR, nucleoplasma separation assay, FISH assay, RIP, dual luciferase reporter assay and rescue assay were used to validate the regulatory effect of circ0093335 on miR-338-5p. The study findings showed that circ0093335 was upregulated in HCC. High circ0093335 expression was linked with the tumour-node-metastasis stage and microvascular tumour invasion. circ0093335 is greatly involved in HCC cell proliferation, aggressive ability and mouse tumour growth, according to many in vitro and in vivo tests. Mechanistically, circ0093335 downregulated miR-338-5p expression by sponging, consequently promoting HCC progression. Our research indicated that circ0093335 might be a target for HCC therapy since it promotes tumour progression by acting as a miR-338-5p 'sponge'.

Relationship between body composition and upper limb physical fitness among Chinese students: 4-Year longitudinal follow-up and experimental study.

Background: Recently, students' fitness has been declining, and high physical fitness level is crucial in establishing optimal physical/mental health and academic performance. The purpose of this study was to explore the relationship between body composition and upper limb physical fitness and the specific aspects of low physical fitness level in Chinese students. Exploring the development and impact factors for upper limb physical fitness can provide a theoretical basis for the health management strategy of students. Methods: Study 1 collected data from 183 male students over 4 years and used Hierarchical Linear Model (HLM) to explore the quadratic predictive role of body composition on upper limb physical fitness. To further explored which aspects of upper limb physical fitness were affected by body composition, study 2 conducted an experimental investigation among 42 male students, comparing different kinds of upper limb physical fitness within two different body composition groups. Results: Studies found (1) from 2015 to 2018, students' Body-Mass-Index (BMI) showed an upward trend, and BMI differences were significant from year to year. While the upper limb physical fitness showed a downward trend. There were significant differences in the number of pull-outs between 2015 and 2016, 2015 and 2017, and 2015 and 2018. (2) The quadratic term of BMI could predict the upper body physical fitness in the same year and the following year. That is, when BMI was medium, the upper body fitness of the same year and the following year was the best. (3) Chinese students with excellent body composition had greater grip strength, drape height and anaerobic power than those with average body composition. Conclusion: In recent years, male students' BMI has been increasing, and the upper body physical fitness has been decreasing. Furthermore, body composition can predict the upper body mass in the same year and the second year, and male students with better body composition also had greater grip strength, drape height and anaerobic power in their upper limbs.

Analysis of Lower Extremity Motor Capacity and Foot Plantar Pressure in Overweight and Obese Elderly Women.

BACKGROUND: Overweight, obesity and falls are major public health problems and old people are the biggest group suffering falls. METHODS: 92 females were divided into the overweight or obesity (O) group (68.85 ± 3.85) and regular-weight (R) group (67.90 ± 4.02). Lower extremity motor capacity and plantar pressure were compared between the two groups. The IRB approval number is 20190804. RESULTS: (1) Functional Movement Screen and Fugl-Meyer Assessment scores in the O group were significantly lower than in the R group. The time to complete the Timed Up and Go test in the O group was significantly longer than in the R group. (2) Foot flat phase, double support distance, and left foot axis angle in the O group were significantly higher than in the R group. Distance and velocity, left-foot minimum subtalar joint angle and right-foot maximum subtalar joint angle in the O group were significantly shorter than in the R group. (3) Peak force, average force and pressure of metatarsal 1-4, mid-foot, heel medial and lateral, peak pressure of metatarsal l, midfoot, heel medial and lateral in the O group were significantly higher than in the R group. (p < 0.05). CONCLUSIONS: Overweight and obese elderly women have a lower sensorimotor function, flexibility and stability in functional movements, but higher loads on the foot.

Relationship Between Mindfulness and Physical Activity in College Students: The Mediating Effect of Eudaimonic Well-Being.

Previous research has shown many benefits of mindfulness for physical and psychological health, including its link to such health behaviors as increased physical activity (PA). We re-examined the association between mindfulness and PA to better understand a possible mediating role of eudaimonic well-being in this association, while controlling for participants' age, gender, and affect. We had 738 undergraduate student participants (45.0% females; M age = 19.58 years) complete measures of trait mindfulness, eudaimonic well-being, and PA, and we used age, gender, and affect as covariates in a single-wave assessment. We conducted this mediation analysis with a bootstrapping technique to produce 10,000 resamples. We replicated findings of a significant relationship between mindfulness and enhanced eudaimonic well-being (ß = 0.474, p < 0.001), which in turn contributed to increased PA (ß = 0.193, p < 0.001). The bootstrapping method indicated a significant indirect effect of eudaimonic well-being (ß = 0.092, 95% CI = [0.043, 0.142]) in the relationship between mindfulness and PA through eudaimonic well-being. Thus, eudaimonic well-being may mediate the connection between mindfulness and PA. College administrators should consider incorporating mindfulness into health promotion programs as a means of enhancing college students' well-being and health behaviors.

Can Physical Exercise Help Deal With the COVID-19 Stressors? Comparing Somatic and Psychological Responses.

This research aims to explore whether physical exercise can buffer the impact of the COVID-19 stressors. Based on the cross-stressor adaptation hypothesis, we proposed a moderated mediation model relating the COVID-19 stressors to sleep disorder via somatic and worry complaints, depending on the amount of physical exercise. A sample of working adults in Beijing (N = 207) filled surveys in two waves during the COVID-19 pandemic. Structural regression analysis showed that physical exercise moderates the impact of the COVID-19 stressors on sleep disorder via somatic complaints (index = -0.11, 95% CI [-0.22, -0.01]), rather than psychological worry complaints (index = -0.01, 95% CI [-0.07, 0.04]). Specifically, the COVID-19 stressors increase somatic complaints for people with a low amount of physical exercise (b = 0.17, p = 0.01]), while the COVID-19 stressors are not significantly related to somatic complaints for people with a high amount of physical exercise (b = -0.06, p = 0.33). This research extends the cross-stressor adaptation hypothesis and provides evidence on an individual intervention of physical exercise to deal with the COVID-19 pandemic.

A Comprehensive Genome Survey Provides Novel Insights into Bile Salt Hydrolase (BSH) in Lactobacillaceae.

Bile salt hydrolase (BSH) is a well-known enzyme that has been commonly characterized in probiotic bacteria, as it has cholesterol-lowering effects. However, its molecular investigations are scarce. Here, we build a local database of BSH sequences from Lactobacillaceae (BSH⁻SDL), and phylogenetic analysis and homology searches were employed to elucidate their comparability and distinctiveness among species. Evolutionary study demonstrates that BSH sequences in BSH⁻SDL are divided into five groups, named BSH A, B, C, D and E here, which can be the genetic basis for BSH classification and nomenclature. Sequence analysis suggests the differences between BSH-active and BSH-inactive proteins clearly, especially on site 82. In addition, a total of 551 BSHs from 107 species are identified from 451 genomes of 158 Lactobacillaceae species. Interestingly, those bacteria carrying various copies of BSH A or B can be predicted to be potential cholesterol-lowering probiotics, based on the results of phylogenetic analysis and the subtypes that those previously reported BSH-active probiotics possess. In summary, this study elaborates the molecular basis of BSH in Lactobacillaceae systematically, and provides a novel methodology as well as a consistent standard for the identification of the BSH subtype. We believe that high-throughput screening can be efficiently applied to the selection of promising candidate BSH-active probiotics, which will advance the development of healthcare products in cholesterol metabolism.

Promotion of Ni2+ removal by masking toxicity to sulfate-reducing bacteria: addition of citrate.

The sulfate-reducing bioprocess is a promising technology for the treatment of heavy metal-containing wastewater. This work was conducted to investigate the possibility of promoting heavy metal removal by the addition of citrate to mask Ni2+ toxicity to sulfate-reducing bacteria (SRB) in batch reactors. SRB growth was completely inhibited in Ni2+-containing medium (1 mM) when lactate served as the sole carbon resource, leading to no sulfate reduction and Ni2+ removal. However, after the addition of citrate, SRB grew well, and sulfate was quickly reduced to sulfide. Simultaneously, the Ni-citrate complex was biodegraded to Ni2+ and acetate. The NiS precipitate was then formed, and Ni2+ was completely removed from the solution. It was suggested that the addition of citrate greatly alleviates Ni2+ toxicity to SRB and improves the removal of Ni2+, which was confirmed by quantitative real-time PCR targeting dissimilatory sulfite reductase (dsrAB) genes. Analysis of the carbon metabolism indicated that lactate instead of acetate served as the electron donor for sulfate reduction. This study offers a potential approach to increase the removal of heavy metals from wastewater in the single stage SRB-based bioprocess.

Presence of Fe3+ and Zn2+ promoted biotransformation of Cd-citrate complex and removal of metals from solutions.

The promotion to Cd-citrate complex biotransformation via addition of Fe(3+) and Zn(2+) was investigated. Single Fe(III)- or Zn-citrate complex was completely degraded by Pseudomonas sp. MBR, Cd-citrate complex was not. In the Cd-citrate media with molar ratio of 1:2 and 1:3, pH increase obtained from the metabolism of excess citrate slightly promoted the biotransformation of Cd-citrate complex, Cd remained in solutions. The presence of Fe(3+) and Zn(2+) resulted in complete biotransformation of Cd-citrate complex in the 1:1:2 Fe:Cd:citrate and Zn:Cd:citrate and 1:1:1:3 Fe:Zn:Cd:citrate media. Alkaline pH obtained from biotransformation of metal-citrate complexes caused almost complete removal of metals (>98%) through precipitation and co-precipitation. Pseudomonas sp. MBR potentially could be used to treat wastewater containing mixed citrate complexes of Fe(III), Zn and Cd.

Simultaneous biodegradation of Ni-citrate complexes and removal of nickel from solutions by Pseudomonas alcaliphila.

The objective of this study was to study the simultaneous biodegradation of Ni-citrate complexes and removal of Ni from solutions by Pseudomonas alcaliphila. Adding excess citrate to 1:1 Ni-citrate complexes promoted the degradation of the complexes and removal of Ni. The alkaline pH generated by the metabolism of excess citrate caused partial dissociation of citrate from the Ni-citrate complexes, allowing degradation, and the released Ni was removed through bioaccumulation and precipitation. Addition of Fe(3+) enhanced the degradation of Ni-citrate complexes and removal of Ni from solutions. The displacement of Ni from recalcitrant Ni-citrate complexes by Fe(3+) and subsequent biodegradation of the degradable Fe(III)-citrate complex resulted in complete metabolism of citrate. The almost complete removal of Ni (>98%) can be attributed to the combination of coprecipitation with Fe(3+), bioaccumulation and precipitation. P. alcaliphila potentially could be applied in the treatment of effluent containing Ni-citrate complexes.

Dissimilatory nitrate reduction by Pseudomonas alcaliphila with an electrode as the sole electron donor.

Denitrification and dissimilatory nitrate reduction to ammonium (DNRA) were considered two alternative pathways of dissimilatory nitrate reduction. In this study, we firstly reported that both denitrification and DNRA occurred in Pseudomonas alcaliphila strain MBR with an electrode as the sole electron donor in a double chamber bio-electrochemical system (BES). The initial concentration of nitrate appeared as a factor determining the type of nitrate reduction with electrode as the sole electron donor at the same potential (-500 mV). As the initial concentration of nitrate increased, the fraction of nitrate reduced through denitrification also increased. While nitrite (1.38 ± 0.04 mM) was used as electron acceptor instead of nitrate, the electrons recovery via DNRA and denitrification were 43.06 ± 1.02% and 50.51 ± 1.37%, respectively. The electrochemical activities and surface topography of the working electrode catalyzed by strain MBR were evaluated by cyclic voltammetry and scanning electron microscopy. The results suggested that cells of strain MBR were adhered to the electrode, playing the role of electron transfer media for nitrate and nitrite reduction. Thus, for the first time, the results that DNRA and denitrification occurred simultaneously were confirmed by powering the strain with electricity. The study further expanded the range of metabolic reactions and had potential value for the recognization of dissimilatory nitrate reduction in various ecosystems.

Autotrophic nitrogen removal from ammonium at low applied voltage in a single-compartment microbial electrolysis cell.

A new approach was developed to achieve autotrophic nitrogen removal from ammonium at low applied voltage in a single-compartment 3-dimensional microbial electrolysis cell (MEC). The MEC consisted of anodic and cathodic electrodes, on which nitrifying and denitrifying biofilms, respectively, were attached. Nitrogen removal can be enhanced at an applied voltage in the MEC. Besides, the nitrogen removal efficiency gradually increased from 70.3% to 92.6% with the increase of applied voltage from 0.2 to 0.4V, as well as the maximum current was varied from 4.4 to 14 mA. The corresponding coulombic efficiency also increased from 82% to 94.4%, indicating that the increasing applied voltage could enhance electron extraction from ammonium during its oxidative removal. The DO was found to be a critical factor which affected the nitrogen removal in this MEC system. These results demonstrated that the MEC process was applicable to achieve autotrophic nitrogen removal from wastewater containing ammonium.